Suharjo, Radix and Sawada, Hiroyuki and Takikawa, Yuichi (2011) Evaluation of PCR - Restriction Fragment Length Polymorphism (RFLP) as a Rapid Identification Method for Japanese Dickeya strains. Japanese journal of phytopathology, 77 (3). p. 246. ISSN 0031-9473
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Abstract
PCR-RFLP of gyrB, rpoD and recA has been suggested as a rapid method for identification of Japanese Dickeya strains (Suharjo et al, 2010). Here, we performed further investigation using this method to identify larger number of Japanese Dickeya strains including all of the MAFF strains deposited as Erwinia chrysanthemi. The result showed that they can be easily divided into 6 groups, namely D. dianthicola, D. dadantii, D. chrysanthemi, D. zeae, new species I, new species II and one strain was identified as Pseudomonas syringae. It was also shown that rice and Setaria (foxtail millet) strains (D. zeae) produced different bands compared to corn strains (D. zeae). Based on the phenotypic characterization, rice and Setaria strains can be differentiated from the other Dickeya strains by inability to utilize malonate. We suggest that these strains may constitute a new biovar of Dickeya spp.
| Item Type: | Article |
|---|---|
| Subjects: | S Agriculture > S Agriculture (General) |
| Divisions: | Fakultas Pertanian (FP) > Prodi Proteksi Tanaman |
| Depositing User: | RADIX SUHA |
| Date Deposited: | 02 Jun 2017 06:37 |
| Last Modified: | 02 Jun 2017 06:37 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/2770 |
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