Suharjo, Radix and Sawada, Hiroyuki and Takikawa, Yuichi (2010) Identification of Japanese Dickeya Strains Using Multi Locus Sequence Analysis (MLSA) and Restriction Fragment Length Polymorphism (RFLP). Japanese Journal of Phytopathology, 76 (3). p. 212. ISSN 0031-9473

japan jurnal phyto vol76 No3.pdf

Download (3MB) | Preview
Official URL:


Although many strains of Dickeya spp (=Erwinia chrysanthemi) have been isolated and identified as pathogens of various plants species in Japan, their taxonomic position have not been fully elucidated. Therefore, we investigated species assignment and developed rapid identification method of those strains. 41 Japanese Dickeya strains isolated from 24 plants species were used in this study. Based on the 16SrDNA, recA, gyrB and rpoD and Rep-PCR (REP, ERIC, BOX) the strains were essentially divided into 6 groups (I – VI). Four of them could be assigned to known Dickeya species, i.e. D. dianthicola (group I), D. dadantii (group III), D. zeae (group IV) and D. chrysanthemi (group V). On the other hand, two of those groups (group II and VI) are suspected to constitute new species. Furthermore, RFLP analysis of recA, gyrB and rpoD genes also enabled us to easily allocate the Dickeya strains to the spesies level.

Item Type: Article
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Proteksi Tanaman
Depositing User: RADIX SUHA
Date Deposited: 02 Jun 2017 06:37
Last Modified: 02 Jun 2017 06:37

Actions (login required)

View Item View Item