Tarigan, Widamay Fresha and Sumardi, Sumardi (2015) Karakterisasi enzim selulase dari bakteri selulolitik Bacillus sp. Proseding seminar nasional sains dan teknologi VI. pp. 736-747.
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Abstract
The aim of this research was to determine the characteristics of cellulose enzyme produced by isolate bacteria of Bacillus sp. isolated from chicken intestine. The experiment was conducted descriptively, by observing the reduced sugar amount produced by the cellulose enzyme activity. The enzyme activity was examined using DNS method. The characteristics of enzyme observed were the period of the optimum production, the optimum temperature, the optimum pH of buffer, and its stability according to the period of the enzyme storage. The results of this experiment showed that the cellulose enzyme produced by Bacillus sp. was optimally manufactured within 12 hours of incubation. The cellulose enzyme could optimally function in the temperatures of 400C and 500C, and performed the highest activity in the temperature of 500C. In its optimum temperature 500C, the enzyme performed the highest activity on pH 4 buffer, and on pH 9 in the temperature of 400C. Cellulose enzyme was found to have a very low level of stability, while being stored in the temperature of 400C.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi |
| Depositing User: | Sumardi Sumardi Sumardi |
| Date Deposited: | 01 Oct 2020 01:05 |
| Last Modified: | 01 Oct 2020 01:05 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/24232 |
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