Handayani, Kusuma and Mubarik, Nisa Racmania and Sudadi, Untung and Santosa, Dwi Andreas ISOLATION AND CHARACTERIZATION OFPHOSPHATE SOLUBILIZING BACTERIA FROM RHIZOSPHEREOF PINNEAPLE PLANTATION IN LAMPUNG, INDONESIA. Plant Archives (International Journal of Plant Research). ISSN 2581-6063 (Submitted)

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Abstract

Cultivation of pineapple (Ananascomosus) on a large scale plantation often use several fertilizers in high dosage. Utilization of rhizosphere microbes such as phosphate solubilizing rhizobacteria (rPSB) was expected to help reducing the dosage of inorganic P fertilizer application due to its capability in dissolving phosphate fixed by Al and Fe. By those, P become more available for plants. This study aims to evaluate the activity of rPSB isolated from pineapple plantation, both in fields/blocks of low and high pineappleproduction, in Lampung province. rBPF was isolated from pineapple plantation blocks with low (R) and high (T) production levels, as well as in the vegetative (V) and generative (G) stages of plant, with 5 replications.The results showed that the PSB population was around 103 CFU/g. Morphological characteristics of PSB isolateswere mostly white, yellowish, gram-positive, and rod-shaped. The optimum pH for average growth was at pH 5.0, while the optimum temperature was at 30°C. The five best isolates from each block were reconfirmed their phosphate solubility ability based on the phosphate solubility index (PSI). The best PSI of isolates in each block were TG3 (0.80), RV5 (1.05), TV1 (1.10), and RG2 (1.20). The increase in the amount of BPF from TV isolates to RV and TG to RG is in line with the increase in available P (Br-P) soil levels from 25.57 to 40.68 and from 23.83 to 25.25 ppm. This indicatesincreasing of PSB activity with the available P level of the soil.Based on 16S rRNA identification, isolate RG2 was similar 99.69 % to Bacillus altitudinis

Item Type: Article
Subjects: Q Science > QR Microbiology
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User: KUSUMA HAN
Date Deposited: 31 May 2020 03:36
Last Modified: 31 May 2020 03:36
URI: http://repository.lppm.unila.ac.id/id/eprint/21241

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