Sumardi, Sumardi and christina, Nugroho Ekowati and Salman, Farisi and Sundari, Ayu Oktalia (2019) Seleksi dan Karakterisasi Bacillus sp. Penghasil Antibakteri Penghambat Vibrio sp. dari Kawasan Hutan Mangrove Hanura. In: Seminar Nasional Sains, Matematika, Informatika dan Aplikasinya (SN-SMIAP) V tahun 2019. (Submitted)

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Abstract

Mangrove forest ecosystem is a place where many special Bacillus sp. found. This study aims to obtain Bacillus sp. isolates that can inhibit the growth of Vibrio sp. This study was conducted by selecting 77 Bacillus sp. isolates. from the Hanura mangrove forest area. In addition, selected bacteria were tested for salt stress, pH stress, pathogenicity test and antibiotic susceptibility test. Selected isolates were cultivated together with Vibrio sp. for 7 days. Bacillus sp. and Vibrio sp. cultured again with the addition of anoxigen photosynthetic bacteria for 4 days. From the results of the initial selection, there were 4 isolates which could inhibit the growth of Vibrio sp. The final results showed that Bacillus IL2K8 isolates were known to inhibit the growth of Vibrio sp., can grow at salinity 0%, 3%, 6% and pH 7 and pH 10. IL2K8 isolates are not pathogenic, resistant to amplicin antibiotics, striptomycin, nalidixic acid and chloramphenicol but sensitive to trimethoprim antibiotics. In the joint culture of liquid media IL2K8 together with Anoxigenic Photosynthetic Bacteria (APB) isolates IL2K8 can inhibit the growth of Vibrio sp.

Item Type: Conference or Workshop Item (Paper)
Subjects: Q Science > QR Microbiology
Depositing User: Sumardi Sumardi
Date Deposited: 04 Nov 2019 08:29
Last Modified: 04 Nov 2019 08:29
URI: http://repository.lppm.unila.ac.id/id/eprint/14957

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