Satria, Heri and Nurhasanah, Nurhasanah and Laila, Aspita and Herasari, Dian (2018) PROSIDING : Impact Ionic Liquid [Emim]OAc to Cellulose Hydrolysis Activities of Indigenous Microorganisms (IMO’s) on Cassava Peels Substrate. In: 5th International Symposium on Innovative Bio-Production Indonesia, 10 Oct 2018, Bogor, Indonesia.

[img] Text
Prosiding ISIBio 2018_3.pdf

Download (8MB)
[img] Text
Isibio-2018.pdf

Download (5MB)

Abstract

An ionic liquid (IL), 1-ethyl-3-methylimidazolium acetate ([Emim]OAc), was used as pretreatment catalyst follow by hydrolysis of cellulose on cassava peels using local selected indigenous microorganisms (IMO’s). Under 25% (w/v) loading mass of substrate and liquid fermentation condition, cellulolytic of cellulase activities and growth of IMO’s were investigated under a series of IL concentration. Optimal temperature and pH were 37C and 5.5 respectively. Enzyme activity was determined by analyzing the hydrolysis of carboxymethylcellulose (CMC) at 37C using the dinitrosalicylic acid. In general, 0.1 M of IL did not inhibit cell growth significantly. In addition, the hydrolytic activities of obtained-cellulases were most active in presence of 0.1 M of IL, while 0.5 M of IL pushed down the activity into an half and 1.0M extremely was losing the activity. The optimum experimental conditions for hydrolysis of pretreated cassava peels using 0.1 M of [Emim]OAc was established at 37 C, for 72 h when yield of glucose rose up to 64.49%.

Item Type: Conference or Workshop Item (Paper)
Subjects: Q Science > QD Chemistry
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Kimia
Depositing User: Dr. Eng. Heri Satria
Date Deposited: 29 Jan 2019 04:17
Last Modified: 29 Jan 2019 04:17
URI: http://repository.lppm.unila.ac.id/id/eprint/10630

Actions (login required)

View Item View Item