Rashidi, NS and Sukmana, Irza and Mataram, Agung and Jawmawati, Noor
SURFACE TREATED AND FIBRIN COATED ELECTROSPUN
POLYACRYLONITRILE FIBER FOR ENDOTHELIAL CELL
GROWTH AND PROLIFERATION.
FACTA UNIVERSITATIS.
(In Press)
Abstract
Polyacrylonitrile (PAN) is a synthetic biocompatible polymer that has been utilized as filtering membrane in hemodialysis and enzyme immobilization purposes. However, its potential usage in other medical applications is limited due to its poor hydrophilicity. To overcome this problem, two groups of electrospun PAN fibers were treated with sodium carbonate (Na2CO3) and sodium hydroxide (NaOH)respectively at 100oC for 5 minutes. Fibrin gel was then coated to the treated samples before seeding with human umbilical vein endothelial cells (HUVECs) for 1 and 3 days. X-ray diffraction results showed increased crystallinity of PAN fibers when treated with Na2CO and NaOH. Contact angle measurements showed that thehydrophilicity of Na2CO3 treated and NaOH treated samples improved from 115° to 88° and 64° respectively. Fourier transform infrared spectroscopy confirmed their hydrophilicity was due to the existence of carboxyl and hydroxyl groups. However, tensile strength of PAN fibers reduced by 34% when treated with Na2CO3 and 42% when treated with NaOH. Cytotoxicity tests showed increased absorbance in day 3 for both treated samples. The absorbance value for NaOH treated PAN fibers maintained until day 7 while Na2CO3
treated PAN fibers showed a slight increase in absorbance
on day 7. In vitro tests showed increased cell adhesion and proliferation after 3 days of culture. PAN treated fibers coated with fibrin are therefore proven to attract
HUVEC cells and promote endothelialization.
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SURFACE TREATED AND FIBRIN COATED ELECTROSPUN
POLYACRYLONITRILE FIBER FOR ENDOTHELIAL CELL
GROWTH AND PROLIFERATION. (deposited 19 Nov 2018 04:15)
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