Rashidi, NS and Sukmana, Irza and Mataram, Agung and Jawmawati, Noor (2018) SURFACE TREATED AND FIBRIN COATED ELECTROSPUN POLYACRYLONITRILE FIBER FOR ENDOTHELIAL CELL GROWTH AND PROLIFERATION. FACTA UNIVERSITATIS, 2018 (3). pp. 1-13. ISSN 2335-0164 (Online)

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Abstract

Polyacrylonitrile (PAN) is a synthetic biocompatible polymer that has been utilized as filtering membrane in hemodialysis and enzyme immobilization purposes. However, its potential usage in other medical applications is limited due to its poor hydrophilicity. To overcome this problem, two groups of electrospun PAN fibers were treated with sodium carbonate (Na2CO3) and sodium hydroxide (NaOH)respectively at 100oC for 5 minutes. Fibrin gel was then coated to the treated samples before seeding with human umbilical vein endothelial cells (HUVECs) for 1 and 3 days. X-ray diffraction results showed increased crystallinity of PAN fibers when treated with Na2CO and NaOH. Contact angle measurements showed that thehydrophilicity of Na2CO3 treated and NaOH treated samples improved from 115° to 88° and 64° respectively. Fourier transform infrared spectroscopy confirmed their hydrophilicity was due to the existence of carboxyl and hydroxyl groups. However, tensile strength of PAN fibers reduced by 34% when treated with Na2CO3 and 42% when treated with NaOH. Cytotoxicity tests showed increased absorbance in day 3 for both treated samples. The absorbance value for NaOH treated PAN fibers maintained until day 7 while Na2CO3 treated PAN fibers showed a slight increase in absorbance on day 7. In vitro tests showed increased cell adhesion and proliferation after 3 days of culture. PAN treated fibers coated with fibrin are therefore proven to attract HUVEC cells and promote endothelialization.

Item Type: Article
Subjects: T Technology > TJ Mechanical engineering and machinery
T Technology > TS Manufactures
Depositing User: Dr. IRZA SUKMANA
Date Deposited: 10 Jul 2019 06:51
Last Modified: 10 Jul 2019 06:51
URI: http://repository.lppm.unila.ac.id/id/eprint/13283

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