Muhartono, Muhartono and Sukohar, Asep and Sutyarso, Sutyarso and Kanedi, Mohammad (2017) Mucoxin (Acetogenin) Induce Expression of Pro-Apoptosis Proteins, Bax and p53, in T47D Breast Cancer Cells. Biomedical & Pharmacology Journal, 10 (2). pp. 641-649.
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Abstract
Though mucoxin has manufactured and widely marketed online, but indepth study of the role of these annonaceous substances on cellular level is still limited.This study aims to find out more about mucoxin anti-cancer effects, especially those associated with the expression of pro-apoptotic proteins, Bax and p53 in T47D breast cancer cell line.The T47D cell lines was divided into four groupsbased on the hour of mucoxin exposure until assaysdone, namely 0, 24, 48, and 72 hours. Concentration levels of mucoxin applied in theexperiment were 0ng/ml, 0.1ng/ml, 0.5ng/ml, 1ng/ml, 5ng/ml and 10ng/ml. The bax gene expression assayed using quantitative PCR (qPCR) methods, whereas protein expression of bax and p53 determined by immunocytochemistry techniques. The results showed that in comparison with the control, both bax and p53 proteins increase by mucoxin treatment.It is suggested that mucoxin worthy classified as a promising anti-cancer drug.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > RB Pathology |
| Divisions: | Fakultas Kedokteran (FK) > Prodi Pendidikan Dokter |
| Depositing User: | SUTYARSO |
| Date Deposited: | 03 Nov 2018 09:13 |
| Last Modified: | 03 Nov 2018 09:13 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/9282 |
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