Ni Luh Gede Ratna Juliasih, Ni Luh Gede Ratna Juliasih and Setiawan, Andi (2018) Cultivation of Nannochloropsis sp in The Media from Effluent Biogas of Tapioca Industrial and Determination of its Protein Content. In: The 2nd International Conference on Applied Sciences Mathematics and Informatics, 9-11 Agustus 2018, Hotel Horison, Bandar Lampung, Indonesia. (Unpublished)

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Abstract

The cultivation of Nannochloropsis sp. in the media from effluent biogas of tapioca industrial (MEBIT) and determination of its protein content has been done. This research included the isolation of Nannochloropsis sp. from marine biota symbiosis with mangrove roots obtained from The Dewi Mandapa coastal, Pesawaran, Lampung and adaptation the inoculum Nannochloropsis sp. in the media MEBIT 1, 3, and 6% enriched with a solution of urea, ZA and TSP. The growth of Nannochloropsis sp. was evaluated based on cell density (OD = Optical Density at 750 nm, the concentration of chlorophyll a and biomass yields, which is compared to the growth in BG 11 media. The results showed that 6% (v/v) MEBIT is appropriate for the growth of Nannochloropsis sp. The biomass concentration of Nannochloropsis sp. in MEBIT is 0.31 g/L with a productivity of 0,019 g Lˉ1 dˉ1 while the media biomass BG 11 gained 0.25 g/L with a productivity of 0,016 g Lˉ1 dˉ1. The protein content of Nannochloropsis sp. growth in MEBIT obtained 30.64% and 36.41% in BG 11 media, of the dry biomass. The verification method for the protein determination used in this research has the precision of 0.015% with the % recovery of 98% and the detection limit of 7.8 mg/mL.

Item Type: Conference or Workshop Item (Speech)
Uncontrolled Keywords: Nannochloropsis sp., effluent biogas, tapioca industry, cultivation media, protein
Subjects: Q Science > QD Chemistry
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Kimia
Depositing User: Dr. Eng. NI LUH GEDE RATNA JULIASIH
Date Deposited: 04 Nov 2018 14:11
Last Modified: 04 Nov 2018 14:11
URI: http://repository.lppm.unila.ac.id/id/eprint/9273

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