Ginting, Cipta and Mujim, Subli and Dianto, A H (2005) Isolasi spesies Verticillium yang berasosiasi dengan Hemileia vastatrix pada daun kopi. Jurnal Natur Indonesia, 8. pp. 114-117. ISSN 14109379

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ABSTRACT The species of Verticillium associating with Hemileia vastatrix on coffee leaves. H. vastarix is an obligate parasite on coffee plant and causes leaf rust that is considered as one of major problems in coffee production. The plant pathogenic fungus was often to be antagonized by Verticillium of unknown species that could be developed as biocontrol agent. The objective of this study was to identify and characterize Verticillium species associated with H. vastatrix on coffee leaves. Leaf samples showing lesions with H. vastatrix uredospores and white mycelium were taken from two coffee fields at Sumberjaya Sub district, West Lampung, and transported in ice chest to the laboratory. Verticillium was isolated with a sterilized needle and grown on PDA-L (PDA supplemented with lactic acid at 1.4 ml l-1). Pure cultures were prepared by hyphal tip method and were kept on PDA slants. For identification and characterization, cultures were grown on PDA-L for 2 weeks under transmitted light at room temperature. Fungal structures were measured using micrometer under a microscope. The average (range) length of phialids was 17 (16–18) µm. The average (range) length and width of conidia were 5.5 (3–9) µm and 1.9 (1.5–2.0) µm, respectively. Based on these and other morphological characteristics, the isolates were tentatively identified as Verticillium lecanii. Three light conditions tested generally did not influence growth and reproduction of V. lecanii. Key words: Verticillium lecanii, Hemileia vastatrix, coffee leaf rust, mycoparasitism

Item Type: Article
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Proteksi Tanaman
Depositing User: Prof Cipta Ginting
Date Deposited: 24 Oct 2016 01:34
Last Modified: 25 Oct 2016 02:02

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