Shinohara, Shinobu and Fitriana, Yuyun and Satoh, Katsuya and Narumi, Issay and Saito, Tsutomu (2013) Enhanced fungicide resistance in Isaria fumosorosea following ionizing radiation-induced mutagenesis. FEMS Microbiology Letters, 349. pp. 54-60. ISSN 1574-6968

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Abstract

The application of entomopathogenic fungi such as Isaria fumosorosea to com-bat insect pests on plants is complicated by their sensitivity to commonly used fungicides. In this study, I. fumosorosea mutants with enhanced resistance tothe fungicide benomyl were induced by irradiation using either ion beams or gamma rays, or a combination of the two. When grown on agar containing benomyl, mycelial growth was observed for five of the six mutant isolates at benomyl concentrations that were more than 2000-fold those observed for the wild-type isolate (EC50: > 5000 mg L-1 c.f. EC50: 2.5 mg L-1 for the wild-type isolate). The mutant isolates evaluated also showed enhanced resistance to other fungicides at recommended field application rates. No differences were observed at the b-tubulin locus between the wild-type and the mutant isolates,suggesting that the enhanced benomyl resistance was not attributable to mutations in that gene. Ion beams and gamma rays are thus potentially useful tools for inducing beneficial fungal mutations and thereby improving the potential for application of entomopathogenic fungi as microbial control agents.

Item Type:	Article
Subjects:	S Agriculture > S Agriculture (General)
Divisions:	Fakultas Pertanian (FP) > Prodi Proteksi Tanaman
Depositing User:	YUYUN FITRIANA
Date Deposited:	03 Nov 2016 04:34
Last Modified:	03 Nov 2016 04:34
URI:	http://repository.lppm.unila.ac.id/id/eprint/855

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