Mahfut, - and Daryono, Budi Setiadi and Indrianto, Ari and Somowiyarjo, Susamto MOLECULAR PHYLOGENY OF MYCORRHIZAL RHIZOCTONIA ISOLATED FROM WILD TROPICAL ORCHIDS IN INDONESIA. Fungal Ecology. (Submitted)

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Official URL: https://www.journals.elsevier.com/fungal-ecology

Abstract

Rhizoctonia sp. has an important role as a biocontrol agent. Its association with Phalaenopsis amabilis was molecularly identified through rDNA-ITS sequence analysis, conducted as one of wild orchid conservation efforts in Indonesia. A group of Rhizoctonia sp. were isolated from the root of orchid plant in Yogyakarta. Verification analysis of these isolates resulted in 600-750 bp DNA products located on the ITS1-5.8S-ITS4 region. The sequenced products showed insertion and substitution occurances, which may result in strain diversity and possible variation in severity. Reconstruction of phylogenetic trees using Maximum Parsimony and Bootstrap-1000 approach showed that Indonesian isolates have undergone speciation and have been positioned in the cluster, which are already far apart from the other isolates. Results of this study were expected to become the basic information in an effort of wild orchid cultivation and protection against infectious diseases in Indonesia. The study was the first to report regarding Rhizoctonia sp. isolated from wild tropical orchids in Indonesia.

Item Type: Article
Uncontrolled Keywords: Rhizoctonia sp.; rDNA-ITS; Phalaenopsis sp.; Orchid Mycorrhizal Fungi.
Subjects: Q Science > QK Botany
S Agriculture > S Agriculture (General)
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User: MAHFUT - -
Date Deposited: 29 Jun 2018 06:38
Last Modified: 29 Jun 2018 06:38
URI: http://repository.lppm.unila.ac.id/id/eprint/8257

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