Setiawan, Kukuh and Muhammad Syamsoel, Hadi and Ardian, . and Timotiwu, P.B. and Agustiansyah, Agustiansyah and Setiawan, A. Wawan and Kamal, Muhammad ROOT YIELD AND STARCH SYNTHASE IV GENE ACTIVITY UNDER DIFFERENT MICRO NUTRIENT FERTILIZER AND HARVEST TIMES ON CASSAVA (Manihot esculenta, Crantz). J. ISSAAS. pp. 1-19. ISSN 0859-3132 (Submitted)

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Abstract

The objectives of this study were to evaluate root fresh weight of cassava applied by micro nutrient fertilizer, to compare yield of root storage treated by micro nutrient fertilizer as 0, 20, and 40 kg/ha, to evaluate the activity of gene starch synthase (SS IV) with Real Time PCR. Treatments were arranged by factorial (3x3) in completely randomized block design with three replications used as block. The first factor was three different dosages of micro nutrient fertilizer as 0, 20, and 40 kg/ha. The second factor was harvest time as 7, 8, and 10 months after planting (MAP). The micro nutrient fertilizer mainly contents of 5,888 ppm Fe and 1,368 ppm Zn. Variables observed in this study were leaf number (LN), leaf fresh weight (LFW), leaf dry weight (LDW), stem fresh weight (SFW), stem dry weight (SDW), fresh root weight (FRW), root dry weight (RDW), skin root fresh weight (SRFW), skin rot dry weight (SRDW), starch content, and the expression of starch synthase gene activity. Starch synthase IV gene activity was observed from the sample taken at 10 MAP of fresh cassava root by Real Time PCR, respectively. The result showed that RDW of cassava applied by 20 kg micro nutrient/ha tended to be the same whether harvested 7, 8 or 10 MAP. Additionally, starch content was increased by high dosages of micro nutrient fertilizer. The increase in RDW was due to mainly high gene SS IV activity. The SS IV gene activity caused by 20 kg micro nutrient/ha treatment showed almost as twice as those by 40 kg micro nutrient/ha. Keywords: harvest time, leaf, micro nutrient, Real Time PCR, root yield, starch, starch synthase (SS IV)

Item Type:	Article
Subjects:	S Agriculture > S Agriculture (General)
Divisions:	Fakultas Pertanian (FP) > Prodi Agronomi dan Hortikultura
Depositing User:	Kukuh Setiawan
Date Deposited:	29 Jun 2018 04:07
Last Modified:	29 Jun 2018 04:07
URI:	http://repository.lppm.unila.ac.id/id/eprint/8195

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