Sumardi, Sumardi and Rochmah, Agustrina and Ekowati, Christina Nugroho and Pasaribu, Yovita Selvie (2018) Characterization of protease from bacillus sp. on medium containing FeCl3 exposed to magnetic field 0.2 mT. IOP Conf. Series: Earth and Environmental Science, 130 (1). pp. 1-12. ISSN 1755-1315

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Official URL: http://iopscience.iop.org/article/10.1088/1755-131...

Abstract

This purpose of this research is to determine the character of the protease enzymes from Bacillus sp. on media content of FeCl3 exposed to 0.2 mT magnetic field. The data obtained were analyzed descriptively. The result showed that protease enzyme without Fe resulted in the highest activity at pH 8, temperature 30°C with the addition of activator Mn2+, and Vmax of 0.28 U/ml, and Km of 4.60 U/ml. The protease enzyme on media without magnetic field exposure and containing Fe yielded the highest activity at pH 8, temperature 30°C with the addition of activator Mn2+, and Vmax of 0.33 U/ml, and Km of 5.64 U/ml. The protease enzyme on medium with magnetic field exposure and use Fe as inductors have the highest activity at pH 9, the temperature of 55° C with the addition of activator Mn2+, and Vmax of 0.35 U/ml, and Km 10.04 U/ml.

Item Type: Article
Subjects: R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Depositing User: Sumardi Sumardi
Date Deposited: 28 Jun 2018 01:58
Last Modified: 28 Jun 2018 01:58
URI: http://repository.lppm.unila.ac.id/id/eprint/7997

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