Niswati, Ainin and Murase, Jun and Kimura, Makoto (2003) Effect of application of rice straw and compost on the community structure of bacteria associated with microcrustaceans in the floodwater of a paddy field microcosm. Soil Science and Plant Nutrition, 49 (2). pp. 191-200. ISSN 0038-0768

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A microcosm experiment was conducted to study the effect of the application of rice straw and compost to a soil on the bacterial communities associated with five species of microcrustaceans in the floodwater of paddy fields based on RFLP analysis. PCR products for bacterial 16S rDNA were digested with four restriction endonucleases (Hinf I, Sau3A I, Hae III, EcoR I). The results showed that the RFLP patterns of the bacterial communities were distinctly different among the microcrustaceans, although DNA fragments common to Tanycypris sp., Cypretta sp., and Heterocypris sp. were recognized. Bacterial communities associated with Tanycypris sp. showed the smallest number of fragments among the microcrustaceans studied. Rice straw and compost application exerted a negligible effect on the band patterns from Cypretta sp. and Moina sp., respectively. Cluster analysis and principal component analysis enabled to separate the band patterns into 2 groups with a total of 4 subgroups: I-1. Moina sp., I-2. Mesocyclops sp., II-1. Tanycypris sp. and Cypretta sp., and II-2. Heterocypris sp. The results indicated that the bacterial association with the microcrustaceans was primarily determined by the genus and species of hosts. Effects of rice straw and compost application on the bacterial communities associated with Moina sp. and Cypretta sp. and the duration of flooding on the bacterial communities associated with all the micro crustaceans were also observed, although the effects were not appreciable compared to those of the kind of host microcrustaceans.

Item Type: Article
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Ilmu Tanah
Depositing User: Prof Dr Ainin Niswati
Date Deposited: 11 Oct 2016 03:39
Last Modified: 11 Oct 2016 03:39

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