Muhartono, Muhartono and Rachmawati, Ermin and Danan Jaya, Bayu Putra (2018) Mucoxin Enhanced Transcription and Protein Expression of P53 in Breast Cancer Cell Line T47D. Pakistan Journal of Biotechnology, 15 (1). pp. 219-224. ISSN 2312-7791

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Abstract

Mucoxin is a type of acetogenin isolated from Rollinia mucosa leaves which is known to inhibit cell proliferation and induce apoptosis. However, the mechanism of mucoxin in regulating and eliminating cancer cells was not clear. This study investigated the mucoxin effect on the transcriptional-translational and posttranslational processes of p53 gene in breast cancer cells line T47D. Breast cancer cells line T47D was divided into three groups referred to hours of assays, namely hour 24th, 48th, 72nd, where each group was given mucoxin with six difference doses, namely 0.1 ng/mL, 0.5 ng/mL, 1 ng/mL, 5 ng/mL and 10 ng/mL with three replicates. Transcription of p53 gene was assayed by quantitative PCR (qPCR), whereas the expresssion of p53 protein assayed by immunocytochemistry. Mucoxin enhance p53 gene and protein in all treatment group. p53 gene transcription increased significantly in 48 h, while expression of p53 protein increased significantly in 72 h. Conclusion: Mucoxin increased transcription of p53 gene and expression of p53 protein on cell line T47D.

Item Type: Article
Subjects: R Medicine > RB Pathology
Divisions: Fakultas Kedokteran (FK) > Prodi Pendidikan Dokter
Depositing User: MUHARTONO
Date Deposited: 21 Jun 2018 02:25
Last Modified: 21 Jun 2018 02:25
URI: http://repository.lppm.unila.ac.id/id/eprint/7122

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