Kustyawati, Maria Erna (2017) Effect of Sub and Supercritical Carbon Dioxide Processing on Microbial Loads in Tempe. In: 9th International Seminar of Indonesian Society for Microbiology (ISISM 9), November 14-15, 2017, Palembang. (Unpublished)

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Abstract

Sub and supercritical carbon dioxide is a non-thermal processing which can be a promising method of shelf life extension for tempeh as it induces microbial inactivation avoiding alterations of its quality attributes. Fresh tempeh was treated using supercritical CO2 (7.6MPa, 35oC) and sub supercritical CO2 (6.3MPa, 25oC) for 5, 10, 15 and 20 min of holding time. Inactivation of mold and bacteria were analyzed, and the color of tempeh was measured. The results showed that there was a significant interaction of pressure and holding times for bacterial and mold inactivation at ρ>0.05. Bacterial populations decreased to about 1.7 Log at supercritical CO2 (7.6 MPa) and 1.08 Log at sub supercritical CO2 (6.3 MPa) while molds decreased to about 4.88 Log and 3.73 Log. The identified bacteria resistance to the supercritical CO2 processing at 7.6 MPa for 20 min was Bacillus sp, and Lactobacillus sp. The molds were more affected by the treatments than the bacteria. However, sub supercritical treatment of 6.3 MPa, 25oC, 10min had less reduction effect on both bacteria and mold of tempeh. The L* value of tempeh treated with sub supercritical CO2 for 10 min was 72.1± 0.9 which was an indication of better color of tempeh compared to that of the L* value of tempeh treated with supercritical CO2. The conclution was that the sub supercritical treatment can be an alternative preservation method for tempeh.

Item Type: Conference or Workshop Item (Speech)
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Teknologi Hasil Pertanian
Depositing User: Dr Maria Erna Kustyawati
Date Deposited: 25 Apr 2018 02:51
Last Modified: 25 Apr 2018 02:51
URI: http://repository.lppm.unila.ac.id/id/eprint/5990

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