Hapsoro, Dwi and Inayah, Titik and Yusnita, Yusnita (2018) Plant Regeneration of sugarcane (Saccharum officinarum L) calli in vitro and its response to gamma irradiation. J. ISSAAS, 24 (1). pp. 58-66. ISSN 0859-3132

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Abstract

One way of mutation breeding in sugarcane is to expose in vitro growing calli to gamma ray, regenerate the calli into plants, and then evaluate the plants . This research sought to study in vitro plant regeneration of sugarcane (Saccharum officinarum L.) calli and to determine LD50 of gamma ray for irradiation of embryogenic calli. The research was conducted at The Plant Laboratory, The University of Lampung, Lampung, Indonesia in 2014. Leafrolls of sugarcane were cultured on callus-inducing (CI) medium containing MS salts, 30 g L-1 sucrose, 100 mg L-1 myo-inositol, 150 m L-1 coconut water, 0.1 mg L-1 thiamine-HCl, 0.5 mg L-1 pyridoxine-HCl, 0.5 mg L-1 nicotinic acid, 2 mg L-1 glycine, and 3 mg L-1 2,4-D. The embryogenic calli were cultured on shoot-inducing (SI) medium which was the same as the CI medium, except that the SI medium used 2.5 mg L-1 benzyladenine and no coconut water. Shoots were rooted on root-inducing (RI) medium containing different concentrations of indolebutyric acid (IBA) (0, 2, 5, 7.5, 10 mg L-1 ). The RI medium was the same as the SI medium except for the plant growth regulators. The most effective IBA concentration for rooting of shoots was 5 mgL-1 . Plantlets with highest number of roots showed highest survival rate (68.4%). A radio sensitivity study by irradiating embryogenic calli with gamma ray (0, 5, 10, 15, 20, 25, 30, 40, 50, 60 Gy) showed that the LD50 was 17 Gy. The irradiated calli were successfully regenerated into plantlets and acclimatized to external environment. Results of these studies could be very useful for mutation breeding of sugarcane.

Item Type: Article
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Divisions: Fakultas Pertanian (FP) > Prodi Magister Agronomi
Depositing User: Prof.Dr.Ir YUSNITA YUSNITA
Date Deposited: 14 Nov 2018 02:45
Last Modified: 14 Nov 2018 02:45
URI: http://repository.lppm.unila.ac.id/id/eprint/5371

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