Nurcahyani, Endang and Sumardi, Sumardi and Yulianty, Yulianty and Tundjung, Tripeni Handayani (2023) Research Based on Molecular Analysis of Cassava Results of Induced Resistance to Fusarium Wilt as a Form of “MBKM” Research Implementation. In: The 4th ULICoSTE 2023, 7-8 September 2023, Lampung.
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2. - LoA ULICoSTE 043--PENELITIAN MBKM 2023.pdf Download (277kB) | Preview |
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3. - RUNDOWN ULICoSTE-2023-PENELITIAN MBKM 2023.pdf Download (219kB) | Preview |
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4. - SERTIFIKAT PRESENTER Prof. Endang Nurcahyani- PENELITIAN MBKM 2023.pdf Download (418kB) | Preview |
Abstract
Cassava (Manihot esculenta Crantz.) is an important food commodity in Indonesia, and in the future this commodity will play an increasingly strategic role in people's lives and the country's economy. Based on the harvested area of food commodities, cassava ranks third after rice and corn, the three of which are the main source of carbohydrates for the community. The center of cassava land in Indonesia is controlled by the Province of Lampung, this situation makes Lampung a supplier of one-third of the national cassava production of the national production of 23.92 million tons. However, there are still many production constraints in cassava cultivation, including Fusarium wilt disease. This disease is caused by Fusarium oxysporum which until now has not been treated effectively. The use of superior cassava cultivars that are resistant to Fusarium wilt with high yields is expected to be an important disease control alternative. Fusaric acid is a toxin produced by the fungus Fusarium oxysporum and is widely used for resistance selection. Disease resistance can be obtained by means of induced resistance. The aims of this study were to analyze: (1) resistance of cassava to Fusarium oxysporum on an in vivo scale in a screen house, it is hoped that resistant cassava will be obtained; (2) peroxidase enzymes activity, which are thought to degrade the epidermis of Fusarium oxysporum; and (3) the pattern of cassava DNA bands, using the RAPD method, it is hoped that the pattern of resistant cassava resistance genes can be identified. This study used a one-factor Completely Randomized Design (CRD), namely concentration of fusaric acid, consisting of 5 levels: 0 ppm, 80 ppm, 100 ppm, 120 ppm, and 140 ppm. Quantitative data for each parameter were analyzed using the Analysis of Variance with a 5% significance level and continued with Tukey's test at 5% significance level. The results showed that: (1) 140 ppm fusaric acid concentration induced the best resistance in cassava plants with resistance criteria and disease intensity of 15%; (2) there was an increase in the activity of the peroxidase enzyme as the concentration of fusaric acid increased, (3) there was a new band of cassava DNA that was resistant to Fusarium oxysporum with a size of 300 bp (OPA_10) which could be predicted as a RAPD candidate marker for cassava resistance to Fusarium oxysporum.
| Item Type: | Conference or Workshop Item (Speech) |
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| Subjects: | Q Science > Q Science (General) |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi |
| Depositing User: | ENDANG NURCAHYANI |
| Date Deposited: | 09 Oct 2023 01:16 |
| Last Modified: | 09 Oct 2023 01:16 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/52589 |
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