Rini, Maria Viva and Suharjo, Radix and Fitriana, Yuyun High phosphorus tolerant mutants of Gigaspora sp. induced by Gamma rays and UV irradiation. In: 9th International Conference on Mycorrhiza, 30th July – 4th August 2017, Prague, Czech Republic.

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Abstract

Gigaspora sp. is one of the Arbuscular Mycorrhiza fungi that are now widely used to im- prove plant growth and health. However, spore germination of this species will be inhib- ited in high phosphorous (P) environment. The aim of this study was to obtain Gigaspora sp. mutants which are tolerant to high P condition using Gamma rays and UV irradiation. The spores of Gigaspora sp. were exposed to Gamma rays with different irradiation dose i.e. 0, 30, 100, 300, 1000, and 3000 gray. Some other spores were exposed to UV light in UV chamber (l=254nm) for 0, 5, 10, 20, 40, and 60 minutes. The treated spores were then put inside cell culture cluster consisted 96 wells which already contained 2000 ppm P solution. Each well was received single spore. The spores were then kept in incubator at temperature of 30oC for 4 weeks after which the number of germinated spore were counted for every treatment. In this study, we obtained 17 mutants (nine from gamma rays and six from UV) which were able to germinate in 2000 ppm of P solution. None of the wild type were germi- nated in the mentioned above P concentration. This result indicated that this Gamma rays and UV irradiation can be used as useful tools to improve survival capability of Gi- gaspora sp. in high phosphorus environment.

Item Type: Conference or Workshop Item (Poster)
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Agroteknolgi
Depositing User: RADIX SUHA
Date Deposited: 10 Nov 2017 16:36
Last Modified: 10 Nov 2017 16:36
URI: http://repository.lppm.unila.ac.id/id/eprint/5232

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