Utomo, Setyo Dwi and Ragiliyani, Ani and Karyanto, Agus (2004) THE EFFECT OF LONGITUDINAL, SLICING OF THE EMBRYONIC AXIS AND GROWTH MEDIA ON SHOOT REGENERATION OF PEANUT IN VITRO. Jurnal Agrotropika, IX (1). pp. 19-24. ISSN 0216-7662
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Abstract
Regeneration in vitro is an essential part of gene transfer system using recombinant DNA technology. The procedure of regeneration in vitro must be efficient. The wounding of explant can enhance the efficiency of plant genetic transformation mediated by Agrobacerium. The objective of this study was to evaluate the effect of slicing of the tip of zygotic embryo axes and culture medium on shoot regeneration of peanut cv. Gajah and Kelinci. A factorial experiment was conducted consisting three factors i.e. slicing the top 1/3 of embryo axes (intact, slicing resulting 2 equal sections, and slicing the tip of axes resulting 4 sectors); culture medium (MS without and with amendment of 6 mg/l benzyl amniopurine (BAP) and 2 mg/l naphtalene acetic acid (NAA); and peanut cultivar (Gajah and Kelinci). One cotyledon was remove from intact seed, then axis with one cotyledon was surface-sterilized. Explants were the top 1/3 of axis without leaflets. The tip of axis was sliced according to the treatments. BAP and NAA increased the number of shoots from intact explants. The slicing of the tip of axis and culturing on medium with BAP and NAA increased the average number of shoots of cv. Gajah but not Kelinci. Slicing resulting 2 equal sections of explants cv. Gajah produced 6.3 shoots per embryo axis, which was higher than slicing 4 equal sectors. Key words: Arachis hypogaea, slicing, tip of embryo axis, BAP, NAA.
| Item Type: | Article |
|---|---|
| Subjects: | S Agriculture > S Agriculture (General) |
| Divisions: | Fakultas Pertanian (FP) > Prodi Agronomi dan Hortikultura |
| Depositing User: | Dr. AGUS KARYANTO |
| Date Deposited: | 02 May 2023 01:40 |
| Last Modified: | 02 May 2023 01:40 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/50854 |
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