Nurcahyani, Endang and Yulianty, Yulianty (2017) In vivo study: Analysis of resistance, the chlorophyll content and the density of stomata of Vanilla planifolia mutant resistant to Fusarium wilt disease. In: ICBS 2017 The 5th International Conference on Biological Sciences, 15-16 september 207, UGM Yogyakarta. (Submitted)
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Abstract
ABSTRACT Research on Vanilla planifolia Andrews plantlet with fusaric acid (FA) has been done before, and found indication of tolerant FA concentration for in vitro resistant plantlet selection. Inoculation of the Fusarium oxysporum f. sp. vanillae isolates (Fov) in resistant plantlets performed in vivo, followed by analysis of DNA and protein profile of mutant. The results of the previous study, in the form of specific DNA bands having a size of 930 bp (OPB_14), 430 bp (OPB_20), and 230 bp; 270 bp (OPD_19), and predicted as RAPD marker candidates for plantlet endurance against Fov; The new protein band (MW ± 18 kD) in SDS-PAGE 1D indicates the formation of plantlet against Fov. The long-term goal in the overall study was to obtain Fov-resistant mutant seedlings. Specific targets to be achieved were resistance testing and characterization of V. planifolia resistant to Fov in vivo. The stages of this research include: analysis of resistance, the chlorophyll content and the density of stomata of mutant resistant to Fusarium wilt disease in vivo. The results showed that in vivo condition using concentration of FA of 110 ppm was effective for suppressing the growing of Fov, by intensity up to 25%, compared to the concentration of 90 ppm and 100 ppm respectively. In other words by using 110 ppm fusaric acid could increased the category criteria to resistant.There was a significant increased in the total of chlorophyll, the chlorophyll a and chlorophyll b, and density of stomata, overall in line with the rising FA concentration. Kata kunci : Mutan Vanilla planifolia; Chlorophyll; Stomata; Fusarium oxysporum f.sp. vanillae, in vivo
| Item Type: | Conference or Workshop Item (Poster) |
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| Subjects: | Q Science > Q Science (General) |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi |
| Depositing User: | ENDANG NURCAHYANI |
| Date Deposited: | 16 Nov 2017 07:36 |
| Last Modified: | 16 Nov 2017 07:36 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/5049 |
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