Dwi, Hapsoro and Doni, Saputra and Yusnita, Yusnita Pengaruh Konsentrasi Benziladenin dan Sukrosa terhadap Multiplikasi Tunas Pisang Raja Bulu (AAB) In Vitro [Effects of Benzyladenine and Sucrose Concentrations on In Vitro Shoot Multiplication of Banana cv. Raja Bulu (AAB Group)]. In: Seminar Nasional Dan Rapat Tahunan Dekan Bidang Ilmu Pertanian BKS-PTN Wilayah Barat., 20-21 July 2017, Universitas Bangka Dan Belitung, Pangkal Pinang. (In Press)

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Abstract

Objectives of this research were to study effects of different benzyladenine and sucrose concentrations on in vitro shoot multiplication of banana cv. Rajabulu, rooting and plantlet acclimatization. An experiment on shoot multiplication was conducted using a completely randomized design with three replicates, each of which consisted of 3 culture bottles with one explant. Treatments were arranged in 5x2 factorial combination, i.e., BA concentrations (0; 1; 2; 4; dan 8 mgL-1), and sucrose concentrations (30 or 50 gL-1). Number of shoot buds and shoots per explant, and length of shoots were observerved after 12 weeks of cultures. Data were subjected to analysis of variance, followed by mean separation using LSD. Results showed that BA concentrations significantly affected number of shoot buds and shoots per explant, but sucrose cncentrations only affected number of shoot buds. The best treatments for shoot bud and shoot multiplication was 2 mgL-1 BA and 50 gL-1 sucrose, which produced 5,2 and 4,2 shoot buds and shoots per explant, respectively. Shoots were successfully rooted and the plantlets were successfully acclimatized in a green house. Key words: In vitro, shoot multiplication, benzyladenine, sucrose, acclimatization.

Item Type: Conference or Workshop Item (Speech)
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Divisions: Fakultas Pertanian (FP) > Prodi Doktor Ilmu Pertanian
Depositing User: Dr. Dwi Hapsoro
Date Deposited: 16 Nov 2017 07:35
Last Modified: 16 Nov 2017 07:35
URI: http://repository.lppm.unila.ac.id/id/eprint/4999

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