Betta, Kurniawan and jhons fatriyadi suwandi, jfs and Hanna Mutiara, Hanna and Aila, Karius (2022) PfCRT GENE POLYMORPHISMS IN PLASMODIUM FALCIPARUM ISOLATES FROM MALARIA PATIENTS IN MALARIA ENDEMIC AREAS USING ACT AS STANDARD THERAPY. Majalah Kedokteran Sriwijaya, 54 (1). ISSN 0852-3835/2685-0486
|
Text
15137-49219-1-PB.pdf Download (969kB) | Preview |
Abstract
The pfcrt gene is a biomarker to determine the resistance of Plasmodium falciparum to chloroquine and amodiaquine. When chloroquine was switched to ACT, it is very likely that there will be an increase in wild type strains (pfcrt K76) due to the absence of exposure to chloroquine. Currently chloroquine has not used for malaria treatment. The aims of this study were to identify polymorphisms in the pfcrt gene and phylogenetic analysis of Plasmodium falciparum isolates from malaria patients in Pesawaran Regency, Lampung Province. This research is a laboratory research by analyzing blood samples which are stored biological materials (BBT). DNA isolation was carried out using a DNA isolation kit QIAmp DNA Mini kit from Qiagen, followed by amplification using an appropriate primer. The amplification results were followed by sequencing and then analyzed using Mega 10. The results showed that all samples had mutations in codons 76 (K76T) and 72 (C72S) of the pfcrt gene. Pesawaran isolates were closely related to other isolates. The conclusion of this study is that polymorphisms were found in codons 72 and 76 of the PfCRT gene, although chloroquine has long been abandoned as an antimalarial. The sequenced pesawaran isolates were also related to other isolates.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Fakultas Kedokteran (FK) > Prodi Pendidikan Dokter |
| Depositing User: | Dr. dr Betta Kurniawan |
| Date Deposited: | 27 Feb 2023 09:14 |
| Last Modified: | 27 Feb 2023 09:14 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/48611 |
Actions (login required)
 |
View Item |