Septiani, Dwi and Nurcahyani, Endang (2022) Salt Stress Resistance of In Vitro Selection Results-Moon Orchid (Phalaenopsis amabilis (L.) Blume). Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati, 9 (2). pp. 12-20. ISSN 2338-4344


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Moon orchid is the one of orchids that most people like because it has a beauty that can be seen on its size, shape, and its colors so that it makes the production of moon orchid becoming increase, but has a problem such as the larger of agricultural land that converted into other sector, the land becomes less productive because of the salt stress or salinity. The one alternative way that efficient and effective to overcome the salt stress is with using varieties that are tolerant to salt stress with selection agent such as NaCl. This research aims to determine the level of of NaCl concentrations that are tolerant to moon orchid and determine the level of resistance of moon orchid with the salt stress by in vitro. The experimental design of this research used completely randomized design with 5 concentration level NaCl 0%; 0,25%; 0,50%; 0,75%; and 1% in Vacin and Went medium. Parameters observed were plantlet percentage, plantlet visualization, height, number of leaves, stomata index, wet weight, chlorophyll content, and salt stress resistance index. The quantitative data from each parameter is analyzed by using Analysis of Variance at 5% significance level and further test with Tukey test at the significant level 5%. The result of this research showed that the concentration level of NaCl 0,25% to 0,50% that tolerated by moon orchid plants by in vitro was in the range of 0,25 - 0,5 categorized by moderate stress, and 0,75% to 1% was in the range 0,5 – 1,0 that categorized with severe stress and the resistance level of the whole concentration in moon orchid is moderate resistance.

Item Type: Article
Subjects: Q Science > Q Science (General)
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Date Deposited: 14 Nov 2022 03:26
Last Modified: 14 Nov 2022 03:26

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