Aeny, Titik Nur and Prasetyo, Joko and Suharjo, Radix and Ratih, Suskandini and Efri, Efri and Saputri, Meri Dwi and Wulandari, Wulandari (2017) ISOLATION AND CHARACTERIZATION OF ACTINOMYCETES FROM PINEAPPLE PLANTATION POTENTIAL AS ANTAGONIST OF DICKEYA SP., THE PINEAPPLE SOFT ROT PATHOGEN. In: International Seminar on Strengthening Food Security ...(Perteta), August, 10-12 2017, Emersia Hotel,Bandar Lampung. (Unpublished)

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Abstract

This study was aimed to isolate and characterize actinomycetes having antagonist activity to Dickeya sp., the pathogen of pineapple soft rot. Soil samples were collected from four different pineapple plantation in Lampung. Samples were air-dried, serially diluted, and plated on actinomycetes isolation agar media; and potential colonies were selected and purified in malt agar medium. Isolates were grouped in different color series based on their aerial-mycelia color, and then morphologically characterized. Antagonist activity of the actinomycetes isolates on Dickeya sp. were evaluated using the same medium. From totally 51 actinomycetes isolates, only 34 isolates that grew well, then screened and tested for antagonistic activity. There were 35 tested isolates (including the control) exhibited a clear zone around the colonies indicating antagonistic activity; only 14 isolates showed clear zone higher than that of the positive control (chloramphenicol). From the smallholder pineapple plantation, isolate A-12 exihibited the highest antagonist activity against Dickeya sp. (diameter of clear zone = 13,69 mm) and from the private pineapple plantation isolate GGP-9 showed the best (diameter of clear zone = 19,44 mm). Based on their cultural and morphological charactyeristics, the isolated actinomyecetes were suggested as genus of Streptomyces. Since many isolates showed inhibitory activity in vitro against Dickeya sp., one of important pineapple soft rot pathogens, it is suggestive that pineapple plantation could be an interesting source to explore for actinomycetes potentially used as antagonist.

Item Type: Conference or Workshop Item (Paper)
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Proteksi Tanaman
Depositing User: Titik Nur Aeny
Date Deposited: 04 Nov 2017 10:46
Last Modified: 04 Nov 2017 10:46
URI: http://repository.lppm.unila.ac.id/id/eprint/4604

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