Farisi, Salman and Setiawan, Wawan Abdullah and Suratman, Suratman (2020) Isolation Of Salmonella typhoid 16s rRNA Gene Fragment Based On Polymerase Chain Reaction (PCR). Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati, 7 (2). ISSN 2338-4344
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3. ISOLATION OF Salmonella typhoid 16s rRNA GENE FRAGMENT BASED ON POLYMERASE CHAIN REACTION (PCR).pdf Download (373kB) | Preview |
Abstract
This study is a further step for the development of a diagnosis of typhoid fever. Through previous research, it was found that typhoid Salmonella bacteria isolates from a sample of patients indicated typhoid fever in Bandar Lampung. Local typhoid Salmonella DNA has been isolated. The maximum quality and quantity of DNA were obtained by adding 3% Polyvinylpyrrolidone (PVP). The next step of DNA analysis becomes easier because the optimal method of isolating typhoid Salmonella DNA has been known. However, for the accuracy of the development of PCR-based molecular markers, species-specific information from the Salmonella typhoid bacterial isolate is required to be used as a template. Information on the specific species of typhoid Salmonella can be determined by performing PCR using 16s rRNA fragments. The highly conserved nature of rRNA makes it possible to synthesize universal primers for PCR processes capable of attaching to conserved sequences of the rRNA genes of the three phylogenic domains: Archaea, Bacteria and Eukarya. PCR is a technique used to amplify nucleic acid sequences using repeated polymerization of DNA sequences. The simplicity and high success rate of amplification of the DNA sequences obtained have made this technique more widely used. In carrying out PCR, the DNA extract, DNA polymerase enzyme, and primer are reacted in a suitable buffer solution. The PCR reaction is carried out with the help of a thermocycle device. Through this research, a single band of local typhoid Salmonella genomic DNA amplification was obtained through PCR using 16s rRNA primers. It is known that the amplicon size is in the range of 1400 bp.
| Item Type: | Article |
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| Subjects: | Q Science > Q Science (General) |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi |
| Depositing User: | WAWAN ABDULLAH SETIAWAN |
| Date Deposited: | 05 Oct 2022 04:06 |
| Last Modified: | 05 Oct 2022 04:06 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/45502 |
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