Hartati, Widya Rini and Sartika, Dewi and Suharyono, Suharyono (2017) Using Ceara Rubber as Natural Anti-Microbe in Reducing Contamination of Staphylococcus aureus, Salmonella sp., Vibrio sp. and Escherichia coli in Mackerel Tuna Fish (Euthynnus affinis). Proceeding International Conference on Cassava.

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Abstract

Mackerel tuna fish is one of fishing results that be like by people because it have thick meat and contain high protein that be source omega 3 fatty acid. However, mackerel tuna fish is easy to be contaminated by microbes, so that causes in reducing quality. Microbe contamination can be reduced by using ceara rubber as an alternative a natural anti-microbe. Ceara rubber contains antimicrobial such as saponin active compound which reduces bacterial cell wall surface tension and inhibits enzyme activity. The objective of this research was to find out the inhibition ability and reduced amount of Staphylococcus aureus, Salmonella sp, Vibrio sp and Escherichia coli bacterias in mackerel tuna fish. This research was conducted in two stages. The first stage was preparing ceara rubber extract samples, bacteria isolation and mackerel tuna fish. The second stage was the research conduct including colony count test, inhibiting zone test and test of reduced counts of Staphylococcus aureus, Salmonella sp, Vibrio sp and Escherichia coli, with 25%. 50%, 75% and 100% ceara rubber extract concentrations. The results showed that ceara rubber extract produced the highest inhibition zone of 13.06 mm at Escherichia coli bacteria, and the followed by 12.78 mm at Salmonella sap, 11.07 mm at Vibrio sap, and the smallest was 10.69 mm at Staphylococcus aureus. The reduced bacteria count results by 104 dilution and ceara rubber extract were 1.68 x 106 cfu/ml to Vibrio sp, 1.41 x 106 cfu/ml to Salmonella sap, 1.3 x 106 cfu/ml to Staphylococcus aureus, and 1.01 x 106 cfu/ml to Escherichia coli.

Item Type:	Other
Subjects:	S Agriculture > S Agriculture (General)
Divisions:	Fakultas Pertanian (FP) > Prodi Teknologi Hasil Pertanian
Depositing User:	Dr Dewi Sartika Syukur
Date Deposited:	12 Sep 2022 01:12
Last Modified:	12 Sep 2022 01:12
URI:	http://repository.lppm.unila.ac.id/id/eprint/44851

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