Nurcahyani, Endang and Andari, Gardis (2021) Analisis Klorofil Planlet Anggrek Tanah (Spathoglottis plicata Bl) Terhadap Fusarium oxysporum. BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research), 8 (2). pp. 58-66. ISSN p-ISSN : 2355-6404 ; e-ISSN : 2685-6360


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Orchids are ornamental plants that are in great demand by Indonesians, their prices are also very high. There are disturbances that occur in the growth of orchids, namely root rot and leaf spot. Fusarium wilt disease caused by Fusarium oxysporum is an important disease because it becomes an obstacle in the quality and production of orchids. The death rate of orchids in the United States caused by Fusarium oxysporum reaches more than 50% of the total number of orchids and this disease is difficult to control using fungicides. One of the efforts to control fusarium wilt disease safely, effectively, efficiently, and without negative impacts is by using high yielding varieties through tissue culture. Tissue culture applications using fusaric acid (AF) are widely used for in vitro selection of many plants. Plants infected with impacting materials will provide a resistance response (Induced resistance) and function to maintain plant survival, especially in warding off the attack of dangerous pathogens. The aim of this study is the specific expression character ofplantlets Spathoglottis plicata resulting from induced resistance to F. oxysporum based on total chlorophyll content, chlorophyll a chlorophyll b. The research method used tissue culture. The overall results of the study on chlorophyll a, clrophil b and total chlorophyll increased and were significantly different. In the control, the chlorophyll a content was 1.722 ± 1.1309E-02, chlorophyll b was 0.585 ± 2.5537E-02 and total chlorophyll was 2.309 ± 2.7503E-02. The chlorophyll a content in the 10 ppm treatment was 2.157 ± 4.7393E-02, the chlorophyll b was 1.470 ± 1.3237E-02, and the total chlorophyll was 3.625 ± 1.0504E-01. The chlorophyll a, b and total content increased in the 20 ppm treatment, namely chlorophyll a 2.834 ± 5.8198E-03, chlorophyll b 2.582 ± 1.8300E-03, total chlorophyll 5.413 ± 8.1599E-03. Chlorophyll a, b and total chlorophyll increased in 30 ppm treatment, namely chlorophyll a 3.297 ± 2.9527E-04, chlorophyll b 3,966 ± 9.1400E-03 and total chlorophyll of 7,258 ± 6.1367E-03. In the 40 ppm treatment, the content of chlorophyll a, b and total chlorophyll increased significantly, namely in chlorophyll a 3.957 ± 1.8898E-02, chlorophyll b 5.642 ± 3.6749E-01 and total chlorophyll of 9.592 ± 2.2269E-01 so it can be concluded that Chlorophyll a, b, and total chlorophyll content in ground orchid plantlet leaves that were resistant to Fusarium oxysporum increased compared to controls with higher concentrations of fusaric acid given

Item Type: Article
Subjects: Q Science > Q Science (General)
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Date Deposited: 25 Jul 2022 01:56
Last Modified: 25 Jul 2022 01:56

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