Sabana, Amalya and Ernawiati, Eti and Priyambodo, Priyambodo and Agustrina, Rochmah (2022) INDUKSI POLIPLOID PLANLET PISANG KEPOK BATU DENGAN PADA MEDIA KULTUR JARINGAN. ORGANISMS: JOURNAL OF BIOSCIENCES, 2 (1). pp. 1-8. ISSN on line ISSN 2808-4012

2022 -(Organisms - Journal of Bioscience - April 2022) - INDUKSI POLIPLOID PLANLET PISANG KEPOK BATU.pdf

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Colchicine is often used in plant breeding to induce mutations and produce polyploid plants with superior traits. Cytologically polylpoid plants have variations in shape, number of chromosomes and larger cell sizes. The use of colchicine in kepok batu bananas is expected to produce polyploid banana kepok batu plants. The purpose of this study was to determine the effect of colchicine compounds on mitotic abnormalities, changes in chromosome number, decreased mitotic index, and the number and length of roots in banana kepok batu plantlets formed on tissue culture media. The research was carried out at the Botanical Laboratory, Department of Biology, FMIPA Unila. This study compared cytology with the squash method between root tip cells of banana kepok batu plantlets after the addition of 0.1% colchicine with controls. Data were analyzed descriptively and displayed in the form of tables and bar charts. The results showed that administration of 0.1% colchicine resulted in mitotic abnormalities (mitotic abnormalities) and increased the number of chromosomes in the control 2n=3x or 33 and 0.1% colchicine treatment, namely 2n=3x+6 or 39. The mitotic index decreased, but averaged – the average number of roots was more and their size was longer than the control. From these results it can be concluded that the administration of 0.1% colchicine was able to induce polyploid cells in banana kepok batu plantlets.

Item Type: Article
Subjects: Q Science > Q Science (General)
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Magister Ilmu Biologi
Depositing User: ROCHMAH AG Rochmah Agustrina
Date Deposited: 12 Jun 2022 11:43
Last Modified: 12 Jun 2022 11:43

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