Susanto, Gregorius Nugroho and Sutyarso, Sutyarso and Supono, Supono and Ikrom, Fadhli Dzil and Hamdani, Asep Yusup (2017) THE EFFECTIVENESS OF USING STEROID EXTRACT OF SEACUCUMBER FOR SEX REVERSAL IN JUVENILE REDCLAW CRAYFISH (Cherax quadricarinatus). In: The 5th International Conference on Biological Sciences (ICBS), 15-16 September 2017, Yogyakarta.

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Abstract

The freshwater crayfish, red claw Cherax quadricarinatus is commercially cultured in many tropical countries. Males of this species have larger harvest size than females, so it is proposed to culture of all-male population for increasing yields. However this practice has hindrance, since it is difficult to provide monosex seed in high quantity.To overcome this problem, it is important to undergo a monosex (single gender) cultivation. Although sex of C. quadricarinatus is determined by genetics, sex reversal from females to males can be triggered by using steroid hormone at their early juvenile stage. The research was conducted to evaluate the effectiveness of sea cucumber's steroid extract for sex reversal from females to males in juvenile crayfish. The research was designed using factorial complete random design. Two different techniques in giving the steroid hormone, which are by immersion and oral. Two groups of 1-2 weeks old juvenile of C. quadricarinatus were immersed in water containing various doses of sea cucumber's steroid extract 2 and 4 mg/L respectively, and the other two groups were administered diet containing either extract of sea cucumber or 17α-methyltestoterone (MT) in dose of 50 mg/kg diets. Sex ratio, survival and growth rate were observed at the end of 50 days juveniles rearing in aquaria. Sex differentiation was determined by visual observation. Data was analyzed by Anova and LSD tests. The results showed that administration of steroid extract of seacucumber was significantly increased the male ratio of crayfish. Treatment dose of 2 mg/L during 18 h immersion was effective to increase male sex percentage from 31.03% (control) to 79.86%. On the other hand, 50 mg/kg of steroid extract of sea cucumber in the diet was more effective to increase the male percentage up to 75,16% than MT. Growth rate of juvenile crayfish, included total length, daily weight gain and biomass, were also significantly improved, while survival rate was insignificant. Thus, the administration steroid extract of seacucumber by both immersion and oral are effective to increase male sex percentage of juvenile crayfish.

Item Type: Conference or Workshop Item (Speech)
Subjects: Q Science > QP Physiology
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Magister Ilmu Biologi
Depositing User: Dr. MSc. Gregorius Nugroho Susanto
Date Deposited: 10 Nov 2017 15:26
Last Modified: 10 Nov 2017 15:26
URI: http://repository.lppm.unila.ac.id/id/eprint/4227

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