Nurcahyani, Endang and Rasmani, Risma and Qudus, Hardoko Insan (2022) In Vitro Selection and Total Phenol Analysis of Moon Orchid [Phalaenopsis amabilis (L.) Bl.] Results of Induced With Fusaric Acid. Advances in Biological Sciences Research, 22 (1). pp. 558-564. ISSN 2581-9615


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Phalaenopsis amabilis (L.) Bl. is an orchid that is included in the list of endangered plant species. The Fusarium wilt disease on P. amabilivirala vital disease caused by Fusarium oxysporum, which of one constraint and up to now days is not well yet managed. Disease control that does not cause negative impacts can be done by using superior cultivars that are resistant to Fusarium oxysporum infection, through in vitro selection on medium with the addition of selective concentration of fusaric acid. The purpose of this study was to determine the concentration of fusaric acid that was tolerant to P. amabilis plantlets and to analyze the total phenol content of plantlets after fusaric acid was induced with various concentrations. This study used a completely randomized design with one factor, with 5 levels of fusaric acid concentration, namely 0 ppm, 30 ppm, 60 ppm, 90 ppm, and 120 ppm. Data analysis used Analysis of Variance with a significance level of 5% and further test with Tukey's test at a significance level of 5%. The results showed that: the concentration of fusaric acid that was tolerant to P. amabilis plantlets was 120 ppm; there was an increase in total phenol content along with an increase in fusaric acid concentration, an increase in total phenol content from 1.54 mg/g at a concentration of 0 ppm (control) increased to 2.0 mg/g at a concentration of 30 ppm, followed by 2.64 mg /g at a concentration of 60 ppm, 3.48 mg/g at 90 ppm and 4.27 mg/g at a concentration of 120 ppm. This proves that the higher the concentration of fusaric acid, the higher the total phenol content produced.

Item Type: Article
Subjects: Q Science > Q Science (General)
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Date Deposited: 17 May 2022 08:27
Last Modified: 17 May 2022 08:27

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