Kanedi, Mohammad and Muhartono, Muhartono and Sutyarso, Sutyarso (2017) Mucoxin (Acetogenin) Induce Expression of Pro-Apoptosis Proteins, Bax and p53, in T47D Breast Cancer Cells. Biomedical & Pharmacology Journal, 10 (2). pp. 641-649. ISSN 2456–2610
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Abstract
Though mucoxin has manufactured and widely marketed online, but indepth study of the role of these annonaceous substances on cellular level is still limited.This study aims to find out more about mucoxin anti-cancer effects, especially those associated with the expression of pro-apoptotic proteins, Bax and p53 in T47D breast cancer cell line.The T47D cell lines was divided into four groupsbased on the hour of mucoxin exposure until assaysdone, namely 0, 24, 48, and 72 hours.Concentration levels of mucoxin applied in theexperiment were 0ng/ml, 0.1ng/ml, 0.5ng/ml, 1ng/ml, 5ng/ml and 10ng/ml. The bax gene expression assayed using quantitative PCR (qPCR) methods, whereas protein expression of bax and p53 determined by immunocytochemistry techniques. The results showed that in comparison with the control, both bax and p53 proteins increase by mucoxin treatment.It is suggested that mucoxin worthy classified as a promising anti-cancer drug.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi |
| Depositing User: | Drs.M.Si. Mohammad Kanedi |
| Date Deposited: | 28 Sep 2017 09:08 |
| Last Modified: | 28 Sep 2017 09:08 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/3976 |
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