Alkausar, Radho and Juliasih, Ni Luh Gede Ratna and Buhani, Buhani and Syafei, Syafei and Nurachman, Zeily and Setiawan, Andi Chromatographic isolation of ß-phycoerythrin from Porphyridium cruentum using ceramic hydroxyapatite. bioflux.

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Abstract

The pigment ß-phycoerythrin is useful as a natural pigment in food and cosmetics, and also serves as a fluoroprobe for laboratory analyses. This study evaluated the effectiveness of ceramic hydroxyapatite as a stationary phase to purify ß-phycoerythrin using medium-pressure liquid chromatography. The microalga, Porphyridium cruentum, was cultivated in artificial sea water consisting of 20% effluent biogas of tapioca waste water, as well as in modified F2 nutrient medium consisting of NaH2PO4 2H2O and NaNO3, stock solution of trace elements (Na2EDTA, NaMoO4 2H2O, CoCl2 6H2O, FeCl3 6H2O, CuSO4 5H2O, ZnSO4 7H2O, MnCl2 4H2O), and stock solution of vitamins (biotin, cyanocobalamin, and thiamine HCl). P. cruentum biomass was harvested after 8 days by centrifuging at 8000 g for 10 min at 4oC. Fresh biomass was resuspended in buffer solution and sonicated for 30 min. Then the slurry was centrifuged at 12000 g for 4 min. ß-phycoerythrin-rich supernatants were pooled and treated as a crude extract. The crude extract was then passed over a ceramic hydroxyapatite column at medium pressure. Ceramic hydroxyapatite was prepared using a precipitation method and sintering at 1200oC. The composition and microstructures of the ceramic were characterized with a scanning electron microscope equipped with an energy dispersive X-ray spectrometer. The purity of ß-phycoerythrin was confirmed using UV-Vis spectroscopy. Ceramic hydroxyapetite offers a scalable method for isolation of ßphycoerythrin. Moreover, effluent biogas of tapioca waste water should be considered as an alternative cultivation medium for sustainable production of microalgae. This study succeeded in cultivating P. cruentum in modified F2 media with 20% biogas waste and utilizing ceramic hydroxyapatite to recover 60% of ß-phycoerythrin with an A546/A280 ratio of 3.995.

Item Type: Other
Subjects: Q Science > Q Science (General)
Depositing User: Dr. Andi Setiawan
Date Deposited: 18 Feb 2022 01:19
Last Modified: 18 Feb 2022 01:19
URI: http://repository.lppm.unila.ac.id/id/eprint/39467

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