Noviany, Noviany and dwi gustia rasyidi, ratu and Setiawan, Andi Antioxidant Assay Guided Separation of the Methanol Stem Extracts of Binahong (Anredera Cordifolia) Using Cyclic Voltammetry Method. Oriental Scientific Publishing Company.

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Abstract

The aim of this study was to identify and characterize groups of antioxidant active naturally from methanol extract of the stem of Anredera cordifolia through antioxidant assay-guided separation. The methanol extract (ME) displayed the highest antioxidant activity, compared to ethyl acetate extract (EE) and hexane extract (HE) using cyclic voltammetry method. Ascorbic acid was used as a positive control in this research. The repeated fractionation of active ME by MPLC and HPLC analyses yielded a constituent with strong antioxidant property. The active constituent (RNV-1) was predicted as a flavonoid type based on the UV spectrum data. The antioxidant activity results exhibited that RNV-1 had a coefficient of antioxidant activity (K) sligtly higher than K of ascorbic acid with the values of 0.055 and 0.052 respectively. It was found that RNV-1 demonstrated an excellent antioxidant activity in comparison with the standard antioxidant activity through antioxidant assays-guided separation. The results obtained suggested that the stem of A. cordifolia can be used potentially as a source of natural antioxidants by contributing beneficial health effects.

Item Type: Other
Subjects: Q Science > Q Science (General)
Depositing User: Dr. Andi Setiawan
Date Deposited: 15 Feb 2022 01:11
Last Modified: 15 Feb 2022 01:11
URI: http://repository.lppm.unila.ac.id/id/eprint/39232

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