Lutfiah, Rosyidatul and Juliasih, Ni Luh Gede Ratna and Hendri, John and Andi, Setiawan Screening Extract EtOAc Sponge Derived-Fungi against Clinical Staphylococcus aureus. International Symposium JESSD 24-25 September. (Unpublished)

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Abstract

The increasing cases of resistance of pathogenic bacteria to various types of antibiotics worldwide has become a serious problem for medicine and human health. This study aims to determine antibacterial activity of extract ethyl acetate (EtOAc) produced by sponge-derived fungi as an antibacterial agent. In this study, nineteen fungal isolates were obtained from the marine sponge in Singaraja, Buleleng Bali, Indonesia. The pathogenic bacteria Staphylococcus aureus was obtained from patients at Abdul Moeloek Hospital, Bandar Lampung. The susceptibility test of Staphylococcus aureus was carried out to nine types of commercial antibiotics using the disk diffusion method. Cultivation and co-cultivation of fungi were carried out by solid state fermentation (SSF) method on rice media. The cultivation and co cultivation results were extracted using EtOAc as a solvent. The bioactivity of the extract was tested against pathogenic bacteria S. aureus. The results of the susceptibility test to antibiotics showed S. aureus resistance to amoxicillin, ciprofloxacin, erythromycin. showed that extracts A12RF, A05RF, C36RF had inhibitory activity against the growth of S. aureus at a concentration of 0,5mg/mL. These results indicate that co-cultivation can induce fungi to produce different secondary metabolites. This basic information is important for further studies related to the production of fungal bioactive compounds through the co-cultivation stage in the SSF process.

Item Type: Article
Subjects: Q Science > Q Science (General)
Depositing User: Dr. Andi Setiawan
Date Deposited: 29 Nov 2021 01:24
Last Modified: 29 Nov 2021 01:24
URI: http://repository.lppm.unila.ac.id/id/eprint/37084

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