Utami, Tya Gita Putri and Juliasih, Ni Luh Gede Ratna and Setiawan, Andi (2021) Screening Antibacterial and Anti-Biofilm Activities of Sponge Derived Actinomycetes against Clinical Isolates of Staphylococcus aureus. In: International Symposium of 2nd JESSD 2021, 25-26 September 2021, Online. (Unpublished)

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Abstract

This study aimed to screen the activity of methanol extract of sponge-derived actinomycetes as an anti-biofilm and antibacterial agent to Staphylococcus aureus. Nine actinomycetes isolates were selected from the UPT LTSIT deposit. S. aureus was obtained from the skin of patients at Abdul Moeloek General Hospital. An antibiotic susceptibility test was performed by the disk diffusion method. Biofilm formation of S. aureus was tested using the crystal violet method. The viability of pathogenic bacteria was measured using the indicator resazurin. The results of the biofilm formation test in vitro revealed that the organic extracts 33A1T2, 33A2T3, 21A1T11, and 38A1T12 inhibited bacterial growth at 0.5 mg/mL. Meanwhile, 50A2T9, 21A1T11, and 38A1T12 significantly inhibited the formation of staphylococcal biofilm on polystyrene at a concentration of 0.25 mg/mL. This information is very important as a basis for further understanding of the mechanism of action of antibiofilm agents.

Item Type:	Conference or Workshop Item (Speech)
Subjects:	Q Science > QD Chemistry
Divisions:	Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Kimia
Depositing User:	Dr. Eng. NI LUH GEDE RATNA JULIASIH
Date Deposited:	14 Nov 2021 05:01
Last Modified:	14 Nov 2021 05:01
URI:	http://repository.lppm.unila.ac.id/id/eprint/36742

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