Priyambodo, Priyambodo and Rustiati, Elly L. and Kurniawati, Yeyen and Putri, Chicka R. and Zulkarnain, D and Arsan, Zulfi and Giyono, Giyono and Widiawati, Aprilia E. and sukatmoko, sukatmoko and Srihanto, Eko Agus (2021) Exploring environmental DNA for Barcoding Analysis of Sumatran Rhino in Way Kambas National Park. In: 2nd ULICOSTE.

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Abstract

The Sumatran rhino (Dicerorhinus sumatrensis) is one of five species of rhino in the world. The existence of the Sumatran rhino is also considered very vulnerable to habitat degradation, internal cross-breeding, disease and hunting. The Sumatran rhino population will experience extinction if there are no mature management measures for the long term. It is assumed that the dynamics of the natural ecosystem in the Sumatran rhino's natural habitat will have a negative effect on the existence of its population. Sumatran rhino puddle water is one of the remaining sources of environmental genetic material. Extraction of eDNA in the puddle water of individual Sumatran rhinos in Sumatra Rhino Sanctuary, Way Kambas National Park aims to determine the results of testing the quality of genetic material from Sumatran rhino puddle water in the Sumatran Rhino Reserve, Way Kambas National Park using simple and molecular methods. DNA extraction using a simple method detects 0/12 samples, whereas using molecular methods can detect 11/12 samples. These results should be followed by detection using specific primers to ensure that the eDNA extracted is Sumatran rhino eDNA

Item Type: Conference or Workshop Item (Paper)
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Depositing User: Priyambodo Priyambodo
Date Deposited: 06 Nov 2021 08:23
Last Modified: 06 Nov 2021 08:23
URI: http://repository.lppm.unila.ac.id/id/eprint/35261

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