Anggreiny, Anggi and Handayani, T.T. and Mahfut, Mahfut and Wahyuningsih, Sri Study of orchid resistance induced by ceratorhiza sp. against orsv infection based on peroxidase activity. Bioscience.

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Bioscience Volume 5 Number 2, 2021 pp. 102-110.pdf

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Abstract

Process of orchid cultivation is often hampered by virus infections. The virus that often infects the orchids is ORSV. Viruses that enter orchid cells and replicate will activate the orchid’s defense response. This defense response is characterized by the increase of peroxidase activity. The orchid defense response can also be activated through induced systemic resistance by inoculation of Ceratorhiza sp. as endophytic mycorrhizae. In this study, Factorial Complete Randomized Design (CRD) was used with 2 factors. The first factor isthe type of orchid (Phalaenopsis amabilis and Dendrobium discolor) and the second factor is the type of treatment (inoculation of mycorrhizae, virus, and mycorrhizae-virus). The orchid’s resistance level is determined by the analysis results of peroxidase activity using spectrophotometer. The results obtained indicate that all treatment combinations strongly influence the increase of peroxidase activity. Peroxidase activity of Phalaenopsis amabilis is 1.42 [(U/mg)/ min] and Dendrobium discolor 1.64 [(U/mg)/ min] in average. Peroxidase activity on Dendrobium discolor was higher than on Phalaenopsis amabilis. This indicates that Dendrobium discolor has a higher level of resistance when compared to Phalaenopsis amabilis

Item Type: Article
Subjects: Q Science > QK Botany
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User: T. T. HANDAYANI
Date Deposited: 06 Nov 2021 02:15
Last Modified: 06 Nov 2021 02:15
URI: http://repository.lppm.unila.ac.id/id/eprint/35116

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