Rahmawati, Selvi (2021) Single Dose Acute Toxicity of Roasted Robusta Coffee Extract (Coffea canephora) in Sprague-Dawley White Rats (Rattus norvegicus) using OECD No. 423 Guideline. In: International Conference on Agromedicine and Medical Sciences (ICAMS) 2021, 26-27 Oktober 2021, Bandar Lampung.

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Abstract

Coffee is one of the most popular drinks in the world. It is known to be beneficial as antioxidant, antibacterial, anti-inflammatory, anticancer and neuroprotective agents. However, excessive coffee consumption has been proven to cause adverse health effects. There is still lack information about the safe dose of coffee consumption. The purpose of this study was to assess the single dose acute oral toxicity of roasted robusta coffee extract in a white rat model using OECD Guideline No. 423. Toxicity tests were conducted to obtain information about the safety and side effects of fixed doses. OECD Method Principle No. 423 is on the use of a stepwise procedure on 3 test animals per stage. The test will proceed to another dose stage based on the results of the first dose test. The test substance was administered orally to a group of experimental animals at a fixed single dose based on OECD No. 423 which are 5, 50, 300, 2000, and 5000 mg/kg BW. The observation includes mortality, symptoms of toxicity and organ function evaluation. Based on the results, it can be concluded that the toxicity test results of a single dose of robusta coffee show that the LD50 value (lethal dose 50) is between 2000 and 5000 mg/kg BW and is in the 5th category of toxicity based on the Global Harmonized System.

Item Type: Conference or Workshop Item (Speech)
Subjects: R Medicine > R Medicine (General)
R Medicine > RB Pathology
Divisions: Fakultas Kedokteran (FK) > Prodi Pendidikan Dokter
Depositing User: Selvi Rahmawati, M.Sc.
Date Deposited: 05 Nov 2021 08:08
Last Modified: 05 Nov 2021 08:08
URI: http://repository.lppm.unila.ac.id/id/eprint/35053

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