Herlinda, Siti and Gustianingtyas, Mimma and Suwandi, Suwandi and Suharjo, Radix and Sari, Jelly Milinia Puspita and Lestari, Ragil Putri (2021) Endophytic fungi confirmed as entomopathogens of the new invasive pest, the fall armyworm, Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae), infesting maize in South Sumatra, Indonesia. Egyptian Journal of Biological Pest Control, 31 (124). pp. 1-13. ISSN 2536-9342

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Abstract

Background: The fall armyworm, Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae), is a new invasive pest in Indonesia causing fnancial losses. S. frugiperda larvae hide in the leaf midrib all day. To overcome the hidden larvae, pathogenic endophytic fungi are needed. The objective of this research was to molecularly identify the endophytic fungal species from South Sumatra and determine the most pathogenic species against S. frugiperda larvae. Endophytic fungal identifcation was based on morphological and molecular characteristics. The molecular identifcation was based on gene sequential analysis of Intergenic Transcribed Spacer (ITS) 1 and ITS 4. Bioassay of the endophytic fungal species was treated against the 2nd larval instar of S. frugiperda. Result: The results of molecular identifcation revealed endophytic fungal species consisted of Chaetomium sp. (GaTpeOi, JgTjPr, PiCrPga, and CaCjPga isolates), Aspergillus niger (L.) Van Tieghem (PsgTjPr, JgByU, and JaBuBys), Beauveria bassiana (Balsamo) Vuillemin (JgSPK, JaGiP, JaSpkPGA(2), JgCrJr, and JaTpOi (1) isolates), Curvularia lunata (Wakker) Boed. (JaGiPRB, CMTJP, JaMsBys, JaSpkPga(3), and JgTgSr isolates), Aspergillus favus Link. (JgPWSR isolate), Penicillium citrinum Thom F 1539 (JaTpOi(2) isolate), and Metarhizium anisopliae (Metschn.) Sorokin (CaTpPGA isolate). All fungal isolates were deposited in the GenBank. The endophytic fungal species of B. bassiana (JgSPK isolate) caused up to (22.67%) mortality, C. lunata (JaSpkPga(3) isolate) caused (17.33%) mortality, and M. anisopliae (CaTpPga isolate) caused (8%) mortality of the pest’s larvae. First report of B. bassiana and C. lunata isolated from maize (Zea mays L.), while M. anisopliae was isolated from red chili (Capsicum annuum L.) as entomopathogenic endophytic fungi against S. frugiperda larvae in Indonesia. Conclusion: The endophytic fungi species of B. bassiana, C. lunata, and M. anisopliae had potentials as entomopathogens of S. frugiperda. Keywords: Endophytic fungi, Invasive pest, Spodoptera frugiperda, Morphological and molecular identifcations, Indonesia

Item Type: Article
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Proteksi Tanaman
Depositing User: RADIX SUHA
Date Deposited: 04 Nov 2021 01:14
Last Modified: 04 Nov 2021 01:14
URI: http://repository.lppm.unila.ac.id/id/eprint/34971

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