Setiawan, Andi and Widyastuti, Widyastuti and irawan, arik and Syahrin Wijaya, Oklis and Laila, Aspita and Setiawan, Wawan Abdullah and Juliasih, Ni Luh Gede Ratna and Nonaka, Kenichi and Arai, Masayoshi and Hendri, John (2021) Fermentation Shrimp Shell Waste in Solid State Using Pseudonocardia carboxydivorans 18A13O1 to Produce Bioactive Metabolites. Fermentation, 7 (247). pp. 1-11. ISSN 23115637


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Marine actinomycetes are prolific microorganisms; however, knowledge of their diver�sity, distribution, and secondary metabolites is limited. Marine actinomycetes represent an un�tapped source of novel bioactive compounds. In this study, we investigated shrimp shell as sub�strates for model production bioactive metabolites from actinomycetes under solid state fermenta�tion (SSF) conditions. A total of fifteen actinomycetes were isolated from six sponges and one tu�nicate. The isolated actinomycetes were grown on solid shrimp shells. Cultures of actinomycetes were extracted with ethyl acetate (EtOAc) and extracts were bioassayed for activity against Staph�ylococcus aureus. One isolate 18A13O1 from the sponge, Rhabdastrella globostellata, exhibited anti�bacterial activity on primary screening compared to the other samples and was chosen for further study. Visualization using SEM showed aerial and substrate mycelia. Through phylogenetic anal�ysis, it was confirmed that isolate 18A13O1 is a Pseudonocardia carboxydivorans. Purification of an EtOAc extract yielded A13B2, which showed a minimum inhibition concentration against S. aureus at 15.6 μg/mL. It can be concluded that this basic information is very important for further studies related to the development of the production of bioactive secondary metabolites through the solid state fermentation process.

Item Type: Article
Subjects: Q Science > Q Science (General)
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Magister Ilmu Kimia
Depositing User: Prof Andi Setiawan
Date Deposited: 01 Nov 2021 01:08
Last Modified: 01 Nov 2021 01:08

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