Saito, Tsutomu and Fitriana, Yuyun and Satoh, Katsuya and Oono, Yukata and Narumi, Issay (2015) Thermotolerant mutants of entomopathogenic fungi obtained by ion beam-and γ ray-induces mutagenesis. Other. Japan Atomic Energy Agency, 2015.
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Yuyun 2013 JAEA.pdf Download (1MB) | Preview |
Abstract
Entomopathogenic fungi, such as Metarhizium anisopliae, are economically important agents for integrated pest management (IPM) programmes of insect pests. However, negative effects of temperature, such as heat stress that exceeds 35 °C, can have serious deleterious effects on conidial germination and persistence, vegetative growth, sporulation, and/or the infection process in these entomopathogenic fungi. For vegetative growth, all the mutants derived from wild-type AcMa5 had an upper thermal limit of 38 °C which was higher than that(36°C) of the wild-type by 2°C. Thermotolerance in conidial germination was evaluated after exposure of conidia to 45±1 °C for 0, 0.5, 1, and 3 h. At 25 and 30 °C, most mutants were as virulent to the maize weevil adults as the wild-type, however, one mutant (PaMa02-ib) almost lost virulence entirely. All mutants had no mutations in the neutral trehalase gene, β-tubulin gene nor ABC transporter gene (ifT1) which were previously associated with thermotolerance and fungicide tolerance in entomopathogenic fungi.
| Item Type: | Monograph (Other) |
|---|---|
| Subjects: | S Agriculture > S Agriculture (General) |
| Divisions: | Fakultas Pertanian (FP) > Prodi Proteksi Tanaman |
| Depositing User: | YUYUN FITRIANA |
| Date Deposited: | 07 Jun 2017 08:43 |
| Last Modified: | 07 Jun 2017 08:43 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/3213 |
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