Suhartati, Tati and Yandri, Yandri (2021) Potential Antibacterial Activity of Bioactive ß-sitosterol from Root Bark of Rhizophora apiculata from Lampung Coastal. Jurnal Kimia Sains dan Aplikasi, 24 (4). pp. 114-119. ISSN ISSN : 1410 8917
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Abstract
ß-sitosterol is an essential bioactive phytosterol naturally present in plant cell membranes. It has a coincident structure with animal cholesterol . This investigation reported isolation, structure analysis, and an antimicrobial assay of ß-sitosterol from the root bark of Bakau Minyak (Rhizophoraapiculata) from Lampung coastal . The isolation of ß-sitosterol was carried out through maceration using methanol, separation by vacuum liquid chromatography (VLC), and purification by column chromatography (CC) using ethyl acetate/n- hexane (2: 8) as eluent. The structure of ß-sitosterol was determined using spectroscopic analysis (UV-Vis, FT-IR,13C-NMR, 1H-NMR, DEPT,and GC-MS). The pure ß-sitosterol has 107.4 mg of white needle crystalline compound, the compound melting point about 140.7 - 141.2°C, the molecular mass confirmed by m/z 414, and UV absorption detected at λ 203.9 nm. The ß-sitosterol antimicrobial bioactivity assay has shown potential activity to be developed as a lead compound against E. colli.
| Item Type: | Article |
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| Subjects: | Q Science > QD Chemistry |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Kimia |
| Depositing User: | Prof. Tati Suhartati |
| Date Deposited: | 27 May 2021 02:34 |
| Last Modified: | 27 May 2021 02:34 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/30803 |
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