Muhartono, Muhartono and subeki, subeki (2016) BREAST ANTICANCER ACTIVITY OF BRUCEIN-A FROM MAKASAR FRUIT (Brucea javanica) AGAINST EXPRESSION OF GENE p53 IN RAT INDUCED DIMETILBENZAANTRAZENA. In: The USR International Seminar on Food Security (UISFS), August 23 - 24, 2016, Bandar Lampung, Indonesia.

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Abstract

Breast cancer is the second leading cause of death after cervical cancer. Treatment of breast cancer is done with radiation, surgery, and chemotherapy which often cause side effects the spread of cancer cells, damaging healthy cells, and mutation. Therefore, it is necessary to find new drugs to treat breast cancer effectively and safely. Previous research showed that brucein-A from makasar fruit (Brucea javanica) had breast anticancer activity in vitro with IC50 0.54 g/L significantly different with standard drug of cisplatin (IC50 0.43 g/L). Encapsulation of brucein-A with liposomes enhance the anticancer activity (IC50 0.39 g/L). Giving encapsulation of brucein-A dose 10 mg/kg bw did not cause damage to the liver and kidneys of rats with SGPT 21.67 IU/L, SGOT 40.67 IU/L, and reduced breast cancer cells in rats. Therefore, the mechanism brucein-A to treat breast cancer cells need to be further investigated in order to develop brucein-A as drug. This study aims to determine the breast anticancer activity of brucein-A from makasar fruit against expression of gene p53 in rat induced dimetilbenzantrazena (DMBA). This research used 27 female rats which were divided into 9 groups. All groups were given the DMBA orally at a dose of 20 mg/kg bw twice a week for 3 weeks in order to form breast cancer in rat. Brucein-A was given orally to each group with dose 0, 2.5, 5, 7.5, 10, 12.5, 15, 17.5, and 20 mg/kg bw once daily for 7 consecutive days. The rats were then maintained for 28 days and fed ad libitum. The results showed that giving brucein-A dose 0, 2.5, 5,7.5, and 10 mg/kg bw in rats induced DMBA give score 1 of p53 gene expression (<25%) with differentiation degree of good.While, the giving of brucein-A dose 12.5, 15, 17.5, and 20 mg/kg bw showed score 3 of p53 gene expression (>75%) with differentiation degree of moderate to bad. Keywords: brucein-A, Brucea javanica, gene p53, makasar fruit

Item Type:	Conference or Workshop Item (Paper)
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisions:	Fakultas Kedokteran (FK) > Prodi Pendidikan Dokter
Depositing User:	Wisnu Satyajaya
Date Deposited:	07 Jun 2017 09:21
Last Modified:	07 Jun 2017 09:21
URI:	http://repository.lppm.unila.ac.id/id/eprint/2827

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