Bahri, Syaiful and Ambarwati, Yuli and Nurhasanah, Nurhasanah Isolation and Identification of Terpenoid Compound from Vetiver Grass-Root (Vetiveria zizanioides Stapf) as a Repellent against Termite (Cyrptotermes sp.) through Bioactivity Assay. Journal of Physics: Conference Series. ISSN Print : 1742-6588 Online : 1742-6596 (Submitted)

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Termites are one of the most problematic pests when they damage structural timber and other materials in structures. Currently, in controlling termite, chemical measures aremost widely used to reduce the infestation of termites. However, several termiticides are containing toxic ingredients such as Copper Naphthenate, CopperAzole, Chromate Copper Arsenate (CCA), and Disodium Octoborate Tetrahydrate (DOT), as well as other active ingredients that have registered under the various brand names around the world. In this study, the terpenoid compounds from vetiver grassroot (Vetiveria zizanioides Stapf) have extracted using n-hexane solvent by the soxhletation method. Separation and purification of fraction B1.3, which is an isolation compound, have produced in the form of colorless oil approximately 0.0225 g by vacuum liquid chromatography (VLC). Of the thin-layer chromatography (TLC) assay using eluents of n-Hexane eluent 100%, n-Hexane : DCM 95%, and n-Hexane : Acetone 70%, a red-purple spot, and Rf values which are 0.27, 0.59, and 0.91 has acquired, respectively. To bioactivity assay as a repellent, it has carried out on acetone solvent,crude extract, and the isolation of the compound. Fourier Transform Infrared (FTIR) and Gas Chromatography-Mass Spectrometry (GCMS) are used to analyze and identify the structure of the isolation compound. The spectrum examination gives an absorption band of N-H stretching vibrations (primer amine) at 3415.30 cm-1 and 3477.16 cm-1 regions that are supported by N-H bending at 1618.56 cm-1 regions. In other areas, it shows the absorption of C-H stretching vibration (short-chain alkanes) at 2924.85 cm-1 that are supported by uptake at 1386.14 cm-1 and 1457.43 cm-1, which is CH absorption of methyl and methylene. Meanwhile, on the mass spectrum, it was obtained a molecular ion peak at M+ = 57.2 (100%)as a base peak and the molecular weight of 87.1 g/mol. All these analyses indicated that the molecular formula is C5H13N, suggesting it is a precursor terpenoid because of C<10 as a single form isoprene unit. Therefore, based on investigated that the proposed structure for fraction B1.3 is a 2-methyl butane-1-amine with the elucidation information. The bioactivity assay of the vetiver grass-root isolation compound was active as a repellent against termite (Cryptotermes sp.). These conclusions designate through parameters of % attractive and attractiveness index (AI), which is an average value of 0.025% and (-0.039), respectively. A negative value of AI indicates the compound effective as a repellent.

Item Type: Article
Subjects: Q Science > QD Chemistry
Depositing User: Dr YULI AMBARWATI
Date Deposited: 18 Nov 2020 02:22
Last Modified: 18 Nov 2020 02:22

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