Juliasih, Ni Luh Gede Ratna and Sagita, Anmalia Dea and Setiawan, Andi and Nurachman, Zeily (2020) Fractination of Fucoxantin from Cyclotella striata using Medium Performance Liquid Chromatography (MPLC). In: ICASMI 2020, 3 September 2020, Bandar Lampung. (Unpublished)
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Abstract
Fucoxanthin has a high value because of its potential as an antioxidant compound that plays an important role in counteracting free radicals, which shown to have anti-inflammatory, anti-tumor, and anti-obesity in humans. Fucoxanthin can be obtained from brown algae and diatoms, one of the sources is C. striata. Cultivation of C. striata was carried out on agricultural fertilizer media, then harvesting was carried out in the stationary phase using the centrifugation method. Extraction of fucoxanthin was carried out with ethanol and fractionation was carried out by Medium Performance Liquid Chromatography (MPLC), while the analysis was carried out using a UV-Vis spectrometer. Fractionation of fucoxanthin from C. striata obtained four fractions, however, asymmetric peaks were seen in fraction 3, which showed a little impurities, and it is confirmed by Thin Layer Chromatography (TLC) analysis, showed elongated stains with Rf 0.56-0.93. Subsequent analysis using a UV-vis spectrophotometer showed a broad peak at a wavelength of 447 nm and a sharp peak at 663 nm which showed the characteristics of fucoxanthin. These studies indicate that the MPLC method can be used for fractionation of fucoxanthin from C. striata.
| Item Type: | Conference or Workshop Item (Speech) |
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| Subjects: | Q Science > QD Chemistry |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Kimia |
| Depositing User: | Dr. Eng. NI LUH GEDE RATNA JULIASIH |
| Date Deposited: | 18 Nov 2020 04:53 |
| Last Modified: | 18 Nov 2020 04:53 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/25928 |
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