Rahmawati, Selvi and Janar Wulan, Anggraini and Utami, Nurul PRELIMINARY STUDY : THE POTENCY OF VEGETABLE COOKING OIL AS ALTERNATIVE CLEARING AGENT FOR HISTOLOGICAL PREPARATION. In: The 1st International Conference on Agromedicine and Medical Science. (Unpublished)
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Abstract
Abstract Purpose: This study aims to explore the potency of vegetable cooking oil as alternative clearing agent for histological preparation. Research Methodology: Two different kind of formaline fixed tissue was taken from 3 rats. Each sample of tissue was cut into approximately 1 cm which were subjected for dehydration in differential alcohol gradients. Later, each cutted tissue was kept in 4 different solution; xylene as original clearing agent, palm oil, corn oil, and coconut oil. Further routine steps of processing, sectioning and staining were done. Those were assessed for gross tissue specimen assessment, staining quality and cellular architecture. Comparison was done among them. Results: This study shows a good overall staining results. Those also shows a good and clear distinct nuclear-cytoplasm and good staining quality which almost equally compared to xylene. The staining intensity also shows a really good result both in nucleus and cytoplasm. Hence, it concludes that all three vegetable cooking oils can be used as alternative clearing agents in histological preparations Limitations: This study is lack of evaluation of stability and longevity of staining after some periods. Contribution: This study is potencial to be used in the field of anatomy, histology, anatomical pathology and other fields related to histological preparation Keywords: xylene, cooking oil, clearing agent, histological preparation
| Item Type: | Conference or Workshop Item (Paper) |
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| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Fakultas Kedokteran (FK) > Prodi Pendidikan Dokter |
| Depositing User: | Selvi Rahmawati, M.Sc. |
| Date Deposited: | 19 Nov 2020 11:36 |
| Last Modified: | 19 Nov 2020 11:36 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/25826 |
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