Yuka, Ramaita Ajizah and Setyawan, Agus and Supono, Supono
(2020)
IDENTIFIKASI BAKTERI PENDEGRADASI TAN (Total Ammonia Nitrogen) DARI TAMBAK UDANG VANAME DI LAMPUNG TIMUR.
Aquatropica Asia.
(Submitted)
Abstract
The increasing of feeding rate in intensive shrimp farming will be trigger the accumulation of
organic matters and toxic compound as a TAN (total ammonia nitrogen) in the pond. One of the
effort to reduce the problem is the using of TAN-degrade bacteria. This research aims to identify
bacteria that potential able to decrase TAN in laboratory scale. This study was held in November
2019 - March 2020. Twelve isolates of bacteria wich isolated from the shrimp pond sediment in
sewage medium at Pasir Sakti, East Lampung. Then the samples will screening to select candidates
for TAN degradation bacteria. The selected bacteria with the highest activity in reducing TAN were
subsequently identified morphologically including cell shape, Gram test, motility, and molecular
identification by 16S rDNA sequence analysis. The result of the research point out that the bacteria
that had highest activity to reduce TAN is T4.10 isolate reached 84,6 % (0,478 to 0,074 mg/L). The morphological, biochemical, and BLAST analysis confirmed that the T4.10 isolate was Bacillus megaterium bacteria with similarity reached 100 %. These bacteria can be used as bioremediation candidates. Shrimp pond waste will be degraded into compounds that can be reused for shrimp
metabolic processes, so the sustainability aquaculture can happened.
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IDENTIFIKASI BAKTERI PENDEGRADASI TAN (Total Ammonia Nitrogen) DARI TAMBAK UDANG VANAME DI LAMPUNG TIMUR. (deposited 17 Nov 2020 04:24)
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